Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor ( CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells. PVRIG may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
Product Name:
Recombinant Human PVRIG (C-mFc)
SKU:
RPES6367
Size:
50μg, 10μg
Species:
Human
Synonyms:
C7orf15, C7orf15MGC138295, CD112R, MGC104322, MGC138297, MGC2463, PVRIG, transmembrane protein PVRIG
Tag:
C-mFc
Sequence:
Thr41-Asp171
Expression Host:
HEK293 Cells
Accession:
Q6DKI7
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Calculated MW:
40.1 kDa
Observed MW:
45-55 kDa
Bio-Activity:
Loaded Biotinylated Human Nectin-2-His on His Biosensor, can bind Human PVRIG with an affinity constant of 84.68 nM as determined in BLI assay.
Form:
Lyophilized powder
Source:
HEK293 Cells-derived Human PVRIG protein Thr41-Asp171, with an C-terminal mFc
Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 ℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
