RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
Product Name:
Human R-Spondin 1/RSPO1 Recombinant Protein (RPES3773)
1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 µg/ml (100 ?l/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml.2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 µg/ml (100 ?l/well) can bind mouse CD36 with a linear ranger of 6.4-800 ng/ml.3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met 1-Ala 263) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
28.2 kDa
AP Mol Mass:
42 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
