ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains; a transmembrane domain; a tyrosine kinase domain; a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain; heart; kidney; in addition to skeletal muscle; parathyroid; cerebellum; pituitary; spleen; testis and breast. And lower levels in thymus; lung; salivary gland; and pancreas. It specifically binds to and is activated by neuregulins; NRG-2; NRG-3; heparin-binding EGF-like growth factor; betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart; the central nervous system and the mammary gland; gene transcription; cell proliferation; differentiation; migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development; and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system; especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation; induction of milk proteins and lactation.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Product Name:
Human/Rhesus HER4/ErbB4 Recombinant Protein (RPES3837)
Measured by its binding ability in a functional ELISA. Immobilized human ErbB4-His at 10 µg/ml (100 ?l/well) can bind biotinylated human NRG1 ; The EC50 of biotinylated human NRG1 is 0.4-0.92 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human ERBB4 (NP_005226.1) (Met1-Arg649) with a C-terminal polyhistidine tag was expressed. Human and Rhesus ERBB4 sequences are identical.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
71.1 kDa
AP Mol Mass:
102 kDa
Formulation:
Lyophilized from sterile PBS; pH7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
