R-spondin-3 (RSPO3); also known as Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2 and CRISTIN1; is a member of the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein and widely expressed in many tissues. RSPO3 contains two Furin-like repeats which have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases; and one TSP type-1 domain; RSPO3 founctions as a activator of the beta-catenin signaling cascade; leading to TCF-dependent gene activation. Otherwise; RSPO3 may negatively regulate the TGF-beta pathway.
Product Name:
Human RSPO3 Recombinant Protein (RPES3051)
Product Code:
RPES3051
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
R-spondin-3,RSPO3,Protein with TSP type-1 repeat, Roof plate-specific spondin-3, Thrombospondin type-1 domain-containing protein 2, PWTSR, THSD2, CRISTIN1
Accession:
Q9BXY4-1
Sequence:
Gln22-His272
Fusion tag:
C-Fc-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human R-spondin-3 is produced by our Mammalian expression system and the target gene encoding Gln22-His272 is expressed with a Fc; 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
56.3 kDa
AP Mol Mass:
61 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
