S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. S100B exerts both intracellular and extracellular functions. Intracellular S100B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation; which might have important implications during brain; cartilage and skeletal muscle development and repair; activation of astrocytes in the course of brain damage and neurodegenerative processes; and of cardiomyocyte remodeling after infarction; as well as in melanomagenesis and gliomagenesis. As an extracellular factor; S100B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental; pro-proliferative or pro-differentiative) depending on the concentration attained by the protein; the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine; presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S100B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma; strokes; and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence; that S100B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence; S100B; far from being a negative determinant of outcome; as suggested previously in the human TBI and ischemia literature; is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage.
Product Name:
Human S100B Recombinant Protein (RPES0764)
Product Code:
RPES0764
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100b, S100 beta, S100 calcium binding protein B,NEF,S100,S100-B,S100beta
Accession:
NP_006263.1
Sequence:
Ser 2-Glu 92
Fusion tag:
N-Fc
Activity:
1.Measured by its ability to bind mouse S100A1 in a functional ELISA.2. Measured by its ability to bind TP53 in a functional ELISA.3. Immobilized recombinant human Fc-S100B at 10 µg/mL (100 ?l/well) can bind biotinylated human S100A1 with a linear range of 15.6-250 ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human S100B (NP_006263.1) (Ser 2-Glu 92) was expressed with the fused Fc region of human IgG1 at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
37.2 kDa
AP Mol Mass:
40 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
