Selenophosphate synthetase 1 (SEPHS1) belongs to the selenophosphate synthase 1 family, Class II subfamily. It has four different isoforms by alternative splicing. Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased, which Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased. SEPHS1 can be activated by phosphate ions and by potassium ions. It can synthesize synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.
Product Name:
Human SEPHS1 Recombinant Protein (RPES4160)
Product Code:
RPES4160
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Selenide, water dikinase 1,Selenium donor protein 1,Selenophosphate synthase 1,SEPHS1,SELD, SPS, SPS1
Accession:
P49903
Sequence:
Met 1-Ser392
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Selenophosphate synthase is produced by our Mammalian expression system and the target gene encoding Met1-Ser392 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
43.9 kDa
AP Mol Mass:
42 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 25mM Tris.HCl,pH7.3,100mM glycine,10% glycerol.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
