Description
Recombinant Human Siglec-3/CD33 Protein
The Recombinant Human Siglec-3/CD33 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Siglec-3/CD33 function and its implications in health and disease.
This product (SKU: RPCB0212) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 53.63 kDa with an observed molecular weight of 70-90 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Recombinant Human Siglec-3/CD33 Protein |
SKU: | RPCB0212 |
Size: | 10 μg , 20 μg , 50 μg , 100 μg |
Reactivity: | Human |
Synonyms: | CD33, SIGLEC-3, SIGLEC3, p67, CD33 molecule, SIGLEC-3, SIGLEC3, p67 |
Tag: | C-hFc&His |
Expression Host: | HEK293 cells |
Calculated MW: | 53.63 kDa |
Observed MW: | 70-90 kDa |
Gene ID: | 945 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Siglec-3/CD33 Protein (RPCB0212), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE. |
Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Human CD33 Protein at 1μg/mL (100 μL/well) can bind CD33 Rabbit pAb with a linear range of 0.03-1.66 ng/mL. |
Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.