SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It's in the SIGLEC (sialic acid binding Ig-like lectin) family and is part of the immunoglobulin superfamily. Monocytic/myeloid-lineage cells express SIGLEC5. It's prevalent in blood leukocytes, the spleen, bone marrow, and at lesser amounts in lymph node; lung; appendix; placenta; pancreas; and thymus. It's also expressed by neutrophils and macrophages, although it isn't found in myelomonocytic cell lines. The SIGLEC5 adhesion molecule is a putative adhesion molecule that functions in sialic-acid dependent cell binding. It binds equally to alpha-2;3- and alpha-2;6- linked sialic acid. The sialic acid recognition site may be hidden by cis interactions between the sialic acids on the same cell surface. Human SIGLEC5 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human SIGLEC5 (O15389) (Met1-Thr 434) was expressed with six amino acids (LEVLFQ) at the C-terminus was expressed and purified.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
47.2 kDa
AP Mol Mass:
67 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
