The Recombinant Human SIRP-alpha/CD172a Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating SIRP-alpha/CD172a function and its implications in health and disease.
This product (SKU: RPCB0297) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 64.10 kDa with an observed molecular weight of 80-100 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human SIRP-alpha/CD172a Protein (RPCB0297), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD47 at 1 μg/mL (100 μL/well) can bind Recombinant Human SIRP alpha with a linear range of 1-6 ng/mL.
Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(RPCB0297) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (RPCB1526) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).
Measured by the ability of the immobilized protein to support the adhesion of the OVCAR-3 cells. The ED 50 for this effect is 0.53-2.12 μg/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The protein is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein.
Recombinant Human SIRP-alpha/CD172a Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human SIRP alpha/CD172a Protein (RPCB0297) was greater than 90% as determined by SEC-HPLC.
Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(RPCB0297) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (RPCB1526) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).
