E3 Ubiquitin-Protein Ligase CHIP is a cytoplasmic protein. The protein CHIP is highly expressed in the muscles, heart, pancreas, brain and placenta. The TPR domain of CHIP interacts with molecular chaperones Hsc70-Hsp70 and Hsp90, causing client substrate ubiquitylation and degradation by the proteasome. CHIP targets misfolded chaperone substrates towards proteasomal degradation. The transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation is mediated by CHIP. CHIP is involved in base-excision repair: it increases the HUWE1/ARF-BP1 dependent monoubiquitination, which causes POLB degradation by the proteasome. It may also modulate receptor stability and activity via the proteasome. Human STUB1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human STUB1 Recombinant Protein (RPES2603)
Product Code:
RPES2603
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
E3 Ubiquitin-Protein Ligase CHIP, Antigen NY-CO-7, CLL-Associated Antigen KW-8, Carboxy Terminus of Hsp70-Interacting Protein, STIP1 Homology and U Box-Containing Protein 1, STUB1, CHIP
Accession:
Q9UNE7
Sequence:
Met 1-Tyr303
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human E3 Ubiquitin-Protein Ligase CHIP is produced by our E.coli expression system and the target gene encoding Met1-Tyr303 is expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
34.9 kDa
AP Mol Mass:
33 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
