Transforming growth factor beta-2 (TGF-beta 2) is a secreted protein which belongs to the TGF-beta family. It is a cytokine that performs several cellular functions and has an essential function in early development. The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. TGF-beta 2 is a glycosylated extracellular protein. TGF-beta 2 has been shown to decrease the activity of interleukin (IL)-dependent T-cell cancers. TGFB2 anomalies can lead to non-syndromic aortic disease (NSAD). Human TGFB2 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 mouse T cells. The ED50 for this effect is 0.3-1.5 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Transforming Growth Factor beta 2 is produced by our Mammalian expression system and the target gene encoding Ala303-Ser414 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
12.7 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 4 mM HCl.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
