Description
Recombinant Human Thy-1/CD90 Protein
The Recombinant Human Thy-1/CD90 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Thy-1/CD90 function and its implications in health and disease.
This product (SKU: RPCB0086) is produced using advanced expression systems and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 13.38 kDa with an observed molecular weight of 25-35KDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Thy-1/CD90 Protein |
| SKU: | RPCB0086 |
| Size: | 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | THY1, Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90 |
| Tag: | N-His |
| Calculated MW: | 13.38 kDa |
| Observed MW: | 25-35KDa |
| Gene ID: | 7070 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Thy-1/CD90 Protein (RPCB0086), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.01 EU/μg of the protein by LAL method |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
CD90, also known as Thy1, is an approximately 25-35 kDa glycoprotein that mediates cellular adhesion and exerts wide ranging effects in different tissues. CD90 is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells. Its species-specific expression in lymphoid cells allows its use as a thymocyte and pan T cell marker in mouse but not in human. CD90 can form dimers and higher order multimers. It binds to heparin, the proteoglycan Syndecan-4, and Integrins alpha M beta 2, alpha V beta 3, and alpha V beta 5. Through these interactions, CD90 inhibits neurite outgrowth, promotes astrocyte adhesion, mediates the adhesion and extravasation of leukocytes and melanoma cells, supports the Thrombospondin-1 induced disassembly of fibroblast focal adhesions, and inhibits the activation of latent TGF-beta 1, myofibroblast differentiation, and lung fibrosis. Human Thy-1 shares 63% and 66% amino acids similarity with mouse and rat homolog.
 | Recombinant Human Thy-1/CD90 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
