TIE-1 (Tyrosine Kinase with Ig and EGF Homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily. These receptors are expressed on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18aa signal peptide, a 727 aa extracellular domain and a 354 aa cytoplasmic domain. so far, two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], but no ligand was found for TIE-1.
Product Name:
Human Tie1 Recombinant Protein (RPES1783)
Product Code:
RPES1783
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Tyrosine-Protein Kinase Receptor Tie-1, TIE1, TIE
Accession:
P35590
Sequence:
Ala22-Gln760
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1 is produced by our Mammalian expression system and the target gene encoding Ala22-Gln760 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
81.0 kDa
AP Mol Mass:
87 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM MES, 150mM NaCl, pH 5.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
