Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Tissue Inhibitor of Metalloproteinases 2 (TIMP-2) is a non N-glycosylated protein with a molecular mass of 22 kDa. It produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
Product Name:
Human TIMP2/TIMP-2 Recombinant Protein (RPES0863)
Product Code:
RPES0863
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Metalloproteinase Inhibitor 2, CSC-21K, Tissue Inhibitor of Metalloproteinases 2,CSC-21K,DDC8
Accession:
P16035
Sequence:
Cys27-Pro220
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Tissue Inhibitor of Metalloproteinases 2 is produced by our Mammalian expression system and the target gene encoding Cys27-Pro220 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
22.8 kDa
AP Mol Mass:
21 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
