Description
Recombinant Human TNFSF13/APRIL/CD256 Trimer Protein
The Recombinant Human TNFSF13/APRIL/CD256 Trimer Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating TNFSF13/APRIL/CD256 Trimer function and its implications in health and disease.
This product (SKU: RPCB1192) is produced using HEK293 cells and features a N-His&Flag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 49.9 kDa with an observed molecular weight of 52-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Recombinant Human TNFSF13/APRIL/CD256 Trimer Protein |
SKU: | RPCB1192 |
Size: | 100 μg |
Reactivity: | Human |
Synonyms: | TNFSF13, APRIL, CD256, TALL-2, TALL2, TNLG7B, TRDL-1, UNQ383/PRO715, ZTNF2 |
Tag: | N-His&Flag |
Expression Host: | HEK293 cells |
Calculated MW: | 49.9 kDa |
Observed MW: | 52-60 kDa |
Gene ID: | 8741 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human TNFSF13/APRIL/CD256 Trimer Protein (RPCB1192), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC. |
Formulation: | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Bio-Activity: | Immobilized Human APRIL at 0.2 μg/mL (100 μL/well), dose response curve for Human BCMA with the EC 50 of 5.2 ng/mL determined by ELISA. |
Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.