The Recombinant Human TNFSF13B/BAFF/CD257 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating TNFSF13B/BAFF/CD257 function and its implications in health and disease.
This product (SKU: RPCB0202) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.04 kDa with an observed molecular weight of 20 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human TNFSF13B/BAFF/CD257 Protein (RPCB0202), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
Please contact us for more information.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 5 μg/mL (100 μL/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL.
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED 50 for this effect is 0.1-1 ng/mL, corresponding to a specific activity of 1×10 6 -1×10 7 units/mg.
Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His (RPCB0376) on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag (RPCB0202) with an affinity constant of 3.05nM as determined in BLI assay (Gator).
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.
Recombinant Human TNFSF13B/BAFF/CD257 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant human BAFF at 5 μg/mL (100 μL/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL.
Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His (RPCB0376) on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag (RPCB0202) with an affinity constant of 3.05nM as determined in BLI assay (Gator).
