Recombinant Human TNFSF9/4-1BB Ligand Trimer Protein
The Recombinant Human TNFSF9/4-1BB Ligand Trimer Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating TNFSF9/4-1BB Ligand Trimer function and its implications in health and disease.
This product (SKU: RPCB0507) is produced using HEK293 cells and features a N-monomeric hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 84.3 kDa with an observed molecular weight of 84 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human TNFSF9/4-1BB Ligand Trimer Protein
High quality, high purity and low endotoxin recombinant Recombinant Human TNFSF9/4-1BB Ligand Trimer Protein (RPCB0507), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Recombinant Human TNFSF9/4-1BB Ligand Trimer Protein was determined by Tris-Bis PAGE under reducing conditions.
Immobilized Human 4-1BB, His Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.3ng/mL determined by ELISA.
