Recombinant Human Transcriptional Repressor Ctcf/CTCF Protein
The Recombinant Human Transcriptional Repressor Ctcf/CTCF Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Transcriptional Repressor Ctcf/CTCF function and its implications in health and disease.
This product (SKU: RPCB0695) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 16.9 kDa with an observed molecular weight of 35-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Transcriptional Repressor Ctcf/CTCF Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Transcriptional Repressor Ctcf/CTCF Protein (RPCB0695), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Transcriptional Repressor CTCF (CTCF) belongs to the CTCF Zinc-Finger Protein family. CTCF contains twelveC2H2-type zinc fingers and interacts with CHD8. CTCF is widely expressed in many tissues, and it is absent inprimary spermatocytes. CTCF is involved in transcriptional regulation by binding to chromatin insulators andpreventing interaction between promoter and nearby enhancers and silencers. CTCF plays an essential role inoocyte and preimplantation embryo development by activating or repressing transcription. In addition, CTCF isalso indispensable in the epigenetic regulation and chromatin remodeling.
Recombinant Human Transcriptional Repressor Ctcf/CTCF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
