Tryptases are Trypsin-like Serine Proteases. β-Tryptases are the main isoenzymes in mast cells. βtryptases form active tetramers with heparin proteoglycan. In the tetramer, the unique arrangement of the active sites facing a narrow central pore, β-Tryptases are resistant to macromolecule protease inhibitors . When mast cells are activated, β-Tryptases are released and participate in provoking inflammatory conditions . β-Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic disorders.
Product Name:
Human Tryptase B-2/TPSB2 Recombinant Protein (RPES1487)
Product Code:
RPES1487
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Tryptase Beta-2, Tryptase-2, Tryptase II, TPSB2, TPS2
Accession:
P20231
Sequence:
Ala19-Pro275
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Tryptase beta-2 is produced by our Mammalian expression system and the target gene encoding Ala19-Pro275 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
29.6 kDa
AP Mol Mass:
30 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
