Description
Recombinant Human Tyrosine-protein kinase receptor UFO/Axl Protein
The Recombinant Human Tyrosine-protein kinase receptor UFO/Axl Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Tyrosine-protein kinase receptor UFO/Axl function and its implications in health and disease.
This product (SKU: RPCB0942) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 71.89 kDa with an observed molecular weight of 100-120 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Tyrosine-protein kinase receptor UFO/Axl Protein |
| SKU: | RPCB0942 |
| Size: | 10 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | AXL, ARK, JTK11, Tyro7, UFO |
| Tag: | C-hFc&His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 71.89 kDa |
| Observed MW: | 100-120 kDa |
| Gene ID: | 558 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Tyrosine-protein kinase receptor UFO/Axl Protein (RPCB0942), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized recombinant Human Gas6 at 2 μg/mL (100 μL/well) can bind Axl,the EC 50 of Axl is 1.77 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Axl receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. Axl functions in dampening the immune response, regulating cytokine secretion, clearing apoptotic cells and debris, and maintaining cell survival. Axl is upregulated in various disease states, such as in the cuprizone toxicity-induced model of demyelination and in multiple sclerosis (MS) lesions, suggesting that it plays a role in disease pathogenesis. Axl expression correlates with poor prognosis in several cancers. Axl mediates multiple oncogenic phenotypes and activation of these RTKs constitutes a mechanism of chemoresistance in a variety of solid tumors. Axl contributes to cell survival, migration, invasion, metastasis and chemosensitivity justify further investigation of Axl as novel therapeutic targets in cancer. The receptor tyrosine kinase AXL is thought to play a role in metastasis. The soluble AXL receptor as a therapeutic candidate agent for treatment of metastatic ovarian cancer. GAS6/AXL targeting as an effective strategy for inhibition of metastatic tumor progression in vivo.
 | Recombinant Human Tyrosine-protein kinase receptor UFO/Axl Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized recombinant Human Gas6 at 2μg/mL (100μL/well) can bind Axl,the EC50 of Axl is 1.77ng/mL. |
