The Recombinant Human Vasorin/SLITL2/VASN Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Vasorin/SLITL2/VASN function and its implications in health and disease.
This product (SKU: RPCB1122) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 62.96 kDa with an observed molecular weight of 80-100 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Vasorin/SLITL2/VASN Protein (RPCB1122), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Vasorin (VASN),a single-pass type I membrane protein, is also known as protein slit-like 2 and SLITL2, which contains one EGF-like domain, ten LRR (leucine-rich) repeats, one LRRCT domain and one LRRNT domain.Vasorin is predominantly expressed in vascular smooth muscle cells, and that its expression is developmentally regulated. vasorin It directly binds to transforming growth factor (TGF)-β and attenuates TGF-β signaling in vitro. This suggests that down-regulation of vasorin expression contributes to neointimal formation after vascular injury and that vasorin modulates cellular responses to pathological stimuli in the vessel wall.
Recombinant Human Vasorin/SLITL2/VASN Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human VASN/SLITL2/Vasorin Protein (RPCB1122) was greater than 95% as determined by SEC-HPLC.
