The Recombinant Human VSIG3/IgSF11 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating VSIG3/IgSF11 function and its implications in health and disease.
This product (SKU: RPCB1551) is produced using HEK293 cells and features a C-hFc&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 50.99 kDa with an observed molecular weight of 60-65 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human VSIG3/IgSF11 Protein (RPCB1551), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human VISTA (RPCB0750) at 5μg/mL (100 μL/well) can bind Biotinylated Human VSIG3/IgSF11 (RPCB1551) with a linear range of 0.02-2.03 μg/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Immunoglobulin superfamily member 11(IGSF11) is expressed on the plasma membrane in the testis and brain. These IGSF proteins undergo final modifications during capacitation and/or the acrosome reaction. IGSF proteins share significant homology with endothelial cell-selective adhesion molecule and coxsackievirus and adenovirus receptor, which mediates cell attachment and homotypic intercellular interactions. In clinical, the IGSF11 has been reported to overexpressed in colorectal cancers and hepatocellular carcinomas, as well as intestinal-type gastric cancers, compared to their corresponding non-cancerous tissues. The IGSF11 has also been found expressed abundantly in the testis and ovary and the IGSF11 can be used as a candidate of cancer-testis antigen.
Recombinant Human VSIG3/IgSF11 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human VISTA Protein at 5μg/mL (100 μL/well) can bind Biotinylated Human VSIG3/IgSF11 with a linear range of 0.02-2.03 μg/mL.
