Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
Product Name:
Human WDYHV1/NTAQ1 Recombinant Protein (RPES3244)
Product Code:
RPES3244
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Protein N-terminal glutamine amidohydrolase,WDYHV1,Protein NH2-terminal glutamine deamidase,N-terminal Gln amidase,Nt(Q)-amidase,C8orf32, NTAQ1
Accession:
Q96HA8
Sequence:
Met 1-Cys205
Fusion tag:
N-GST
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Protein N-terminal glutamine amidohydrolase is produced by our E.coli expression system and the target gene encoding Met1-Cys205 is expressed with a GST tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
49.8 kDa
AP Mol Mass:
45-50 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of PBS,100mM GSH,1% TritonX-100,15% Glycerol,pH7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
