Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein
The Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Recombinant Human5'-Nucleotidase/NT5E/CD73 function and its implications in health and disease.
This product (SKU: RPCB0377) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 61.10 kDa with an observed molecular weight of 60-70 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein (RPCB0377), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human 5-Nucleotidase/CD73 at 2 μg/mL (100 μL/well) can bind 5-Nucleotidase/CD73 Rabbit pAb with a linear range of 0.16-2.93 ng/mL.
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >33654 pmol/min/μg.
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is >25,000 pmol/min/μg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The proteins are used as determinants of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries.
Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human 5'-Nucleotidase/CD73 Protein (RPCB0377) was greater than 90% as determined by SEC-HPLC.
