Agouti Related Protein (AGRP, or AGRT), is an endogenous antagonist of the melanocortin receptors MC3R and MC4R found in the hypothalamus and exhibits potent orexigenic activity. AGRP can act as a competitive antagonist to proopiomelanocortin (POMC)-derived peptides at the melanocortin-4 receptor (MC4R), and that this homeostatic mechanism is important as a means of coordinating appetite with perceived metabolic requirement. AGRP is upregulated by fasting while intracerebroventricular injections of synthetic AGRP lead to increased appetite and food intake. Thus, AGRP is a powerful orexigenic peptide that increases food intake when ubiquitously overexpressed or when administered centrally.
Product Name:
Mouse AgRP/AGRP Recombinant Protein (RPES3238)
Product Code:
RPES3238
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
Agrt,Art
Accession:
NP_031453.1
Sequence:
Met1-Thr131
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse AGRP (Met1-Thr131) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
> 85 % as determined by SDS-PAGE
Mol Mass:
39.4 kDa
AP Mol Mass:
41 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
