ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.
Product Name:
Mouse Autotaxin/ENPP2 Recombinant Protein (RPES0389)
Product Code:
RPES0389
Size:
10µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
ATX,E-NPP 2,lysoPLD,Npps2,PD-Ialpha,Pdnp2
Accession:
Q9R1E6-1
Sequence:
Ser 49-Ile 862
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of mouse ENPP2 (Q9R1E6-1) (Ser 49-Ile 862) was fused with a polyhistidine tag at the N-terminus.
Purity:
> 93 % as determined by SDS-PAGE
Mol Mass:
96 kDa
AP Mol Mass:
55-75 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
