Description
Recombinant Mouse B7-H5/Gi24/VISTA Protein
The Recombinant Mouse B7-H5/Gi24/VISTA Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate B7-H5/Gi24/VISTA biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1364) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 18.59 kDa with an observed molecular weight of 35-45 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse B7-H5/Gi24/VISTA Protein |
| SKU: | RPCB1364 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Mouse |
| Synonyms: | B7-H5, Gi24, VISTA, SISP1, VISTA |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 18.59 kDa |
| Observed MW: | 35-45 kDa |
| Gene ID: | 74048 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse B7-H5/Gi24/VISTA Protein (RPCB1364), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA.Immobilized Mouse B7-H5/VISTA (RPCB1364) at 0.5 μg/mL (100 μL/well) can bind B7-H5/VISTA Rabbit pAb with a linear range of 0.01-2.6 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
VSIR, V-Set Immunoregulatory Receptor, also known as VISTA, is an immunoregulatory receptor that inhibits the T-cell response. It may promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. VSIR, or V-set immunoregulatory receptor, could be involved in the pathogenesis of chronic rhinosinusitis with nasal polyps. V-domain Immunoglobulin Suppressor of T cell Activation (VISTA) is an inhibitory immune-checkpoint molecule that suppresses CD4+ and CD8+ T cell activation when expressed on antigen-presenting cells. VSIR is broadly expressed in the spleen, bone marrow, and other tissues. Diseases associated with VSIR include Ichthyosis, Congenital, Autosomal Recessive 6, and Monckeberg Arteriosclerosis.
 | Recombinant Mouse B7-H5/Gi24/VISTA Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized Mouse B7-H5/VISTA (RPCB1364) at 0.5 μg/mL (100 μL/well) can bind B7-H5/VISTA Rabbit pAb with a linear range of 0.01-2.6 ng/mL. |
