Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
Product Name:
Mouse CA10 Recombinant Protein (RPES1744)
Product Code:
RPES1744
Size:
100 µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
2700029L05Rik,BB085816,Ca10,Car10,RP23-448I9.1
Accession:
P61215
Sequence:
Met 1-Asn 300
Fusion tag:
C-His
Activity:
Measured by its esterase activity.The specific activity is >10 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse Car10 (P61215) (Met 1-Asn 300) was expressed, fused with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
33.1 kDa
AP Mol Mass:
38 kDa
Formulation:
Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
