Mouse CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity.CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
Product Name:
Mouse CD73/NT5E Recombinant Protein (RPES0420)
Product Code:
RPES0420
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
5'-nucleotidase,Ecto-5'-nucleotidase,CD73,5'-NT,AI447961, eNT, NT, Nt5 Protein
Accession:
NP_035981.1
Sequence:
Trp29-Phe550
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp29-Phe550 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
58.8 kDa
AP Mol Mass:
60 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
