The Recombinant Mouse CXCL4/PF-4 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate CXCL4/PF-4 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1884) is produced using advanced expression systems and features a C-hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 34.17 kDa with an observed molecular weight of 40-45 kDa under denaturing conditions, achieving ≥ 90% as determined by SDS-PAGE;≥ 90% as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse CXCL4/PF-4 Protein (RPCB1884), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 90% as determined by SDS-PAGE;≥ 90% as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CCL5 (Catalog: RP01750LQ) at 5 μg/mL (100 μL/well) can bind Mouse CXCL4(RPCB1884) with a linear range of 10-252.6 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Recombinant Mouse CXCL4/PF-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Mouse CXCL4 is greater than 90% as determined by SEC-HPLC.
