Description
| Product Name: | Recombinant Mouse Dkk-3 Protein |
| SKU: | RPCB1896 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Mouse |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse Dkk-3 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.01EU/μg of the protein by LAL method. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 50781 |
DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | Q9QUN9 |
 | Recombinant Mouse Dkk-3 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-50kDa. |
 | Recombinant Mouse Dkk-3 inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 9.66-38.64 μg/mL, corresponding to a specific activity of 2.59×101~1.04×102 units/mg. |
