The Recombinant Mouse Dkk-3 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Dkk-3 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1896) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 37.14 kDa with an observed molecular weight of 55-70 kDa under denaturing conditions, achieving ≥ 90% as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Mouse Dkk-3 Protein
SKU:
RPCB1896
Size:
10 μg , 20 μg , 50 μg , 100 μg
Reactivity:
Mouse
Synonyms:
Dkk3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, mDkk-3
High quality, high purity and low endotoxin recombinant Recombinant Mouse Dkk-3 Protein (RPCB1896), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 90% as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED 50 for this effect is 9.66-38.64 μg/mL, corresponding to a specific activity of 2.59×10 1 ~1.04×10 2 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.
Recombinant Mouse Dkk-3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse Dkk-3 inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 9.66-38.64 μg/mL, corresponding to a specific activity of 2.59×101~1.04×102 units/mg.
