The Recombinant Mouse EGF Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate EGF biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1323) is produced using Pichia and features a No-tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 6.45 kDa with an observed molecular weight of 11 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse EGF Protein (RPCB1323), tested reactivity in Pichia and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED 50 for this effect is 37-148 pg/mL, corresponding to a specific activity of 7.14×10 6 ~2.50×10 7 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland.
Recombinant Mouse EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 37-148 pg/mL, corresponding to a specific activity of 7.14×106~2.50×107 units/mg.
