Description
Recombinant Mouse EGFR/HER1 Protein
The Recombinant Mouse EGFR/HER1 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate EGFR/HER1 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1995) is produced using HEK293 cells and features a C-6His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 70.60 kDa with an observed molecular weight of 100-130 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse EGFR/HER1 Protein |
| SKU: | RPCB1995 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Mouse |
| Synonyms: | Egfr, Epidermal growth factor receptor |
| Tag: | C-6His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 70.60 kDa |
| Observed MW: | 100-130 kDa |
| Gene ID: | 13649 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse EGFR/HER1 Protein (RPCB1995), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Recombinant Mouse EGFR/HER1 Protein captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 9.96 nM as determined in SPR assay (Biacore T200). |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
 | Recombinant Mouse EGFR/HER1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | The purity of Mouse EGFR is greater than 95% as determined by SEC-HPLC. |
