Fibroblast growth factor receptor-like 1 (FGFRL1) also known as Fibroblast growth factor receptor 5 (FGFR5), is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A unique feature of FGFRL1/FGFR5 is that it does not contain an intracellular tyrosine kinase domain. Some muscle types, including the muscles of the tongue and the diaphragm, express FGFRL1/FGFR5 at relatively high level. In contrast, the heart and the skeletal muscles of the limbs, as well as many other organs (brain, lung, liver, kidney, gut) express Fgfrl1 only at basal level. It is conceivable that FGFRL1/FGFR5 interacts with other Fgfrs, which are expressed in cartilage and muscle, to modulate FGF signaling.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Product Name:
Mouse FGFRL1/FGFR5 Recombinant Protein (RPES3508)
Product Code:
RPES3508
Size:
100 µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
FGFR5,FGFR5beta,FGFR5gamma
Accession:
NP_473412.1
Sequence:
Met 1-Pro 374
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.1. Immobilized mouse at 10 µg/ml (100 ?l/well) can bind mouse FGFR5. The EC50 of mouse FGFR5 is 0.34 µg/ml.2. Immobilized human FGF1 at 10 µg/ml (100 ?l/well) can bind mouse FGFR5 with a linear range of 0.08-2 µg/ml.3. Immobilized human bFGF at 5 µg/ml (100 ?l/well) can bind mouse FGFR5. The EC50 of mouse FGFR5 is 0.22 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse FGFRL1 (NP_473412.1) (Met 1-Pro 374) precursor was expressed, with a C-terminal polyhistidine tag.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
40.4 kDa
AP Mol Mass:
55-60 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
