The Recombinant Mouse FSTL3 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate FSTL3 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB2198) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 26 kDa with an observed molecular weight of 35-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Mouse FSTL3 Protein
SKU:
RPCB2198
Size:
100 μg
Reactivity:
Mouse
Synonyms:
Follistatin-like protein 3, FLRG, Follistatin-like 3, FSTL3
High quality, high purity and low endotoxin recombinant Recombinant Mouse FSTL3 Protein (RPCB2198), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Bio-Activity:
Immobilized Human Activin A, No Tag at 0.2 μg/mL (100 μL/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC 50 of 18.0 ng/mL determined by ELISA. 2. Mouse FSTL3, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A, No tag with an affinity constant of 53.09 pM as determined in SPR assay (Biacore T200). 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED 50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
Recombinant Mouse FSTL3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Mouse FSTL3 is greater than 95% as determined by SEC-HPLC.
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
