The Recombinant Mouse IgE Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IgE biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB2070) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 38.99 kDa with an observed molecular weight of 45-75 kDa under denaturing conditions, achieving ≥ 95% as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Mouse IgE Protein
SKU:
RPCB2070
Size:
50 μg , 100 μg
Reactivity:
Mouse
Synonyms:
IgE
Tag:
C-His
Expression Host:
HEK293 cells
Calculated MW:
38.99 kDa
Observed MW:
45-75 kDa
Protein Description:
High quality, high purity and low endotoxin recombinant Recombinant Mouse IgE Protein (RPT0013), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95% as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse IgE (Catalog: RPT0013) at 1 μg/mL (100 μL/well) can bind Mouse IgE Rabbit mAb(Catalog: A23195) with a linear range of 0.06-0.45 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
As one of the five designated immunoglobulin isotypes, immunoglobulin E (IgE) plays a major role in atopic conditions by inducing immediate hypersensitivity reactions. IgE also contributes significantly to the body’s immune response to parasitic infections. IgE antibodies are predominantly found in the tissues, firmly attached to effector cells, such as mast cells and basophils, by high-affinity IgE Fc receptor (Fc epsilon RI) and low-affinity IgE receptor (Fc epsilon RII).
Recombinant Mouse IgE Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Mouse IgE (Catalog: RPT0013) at 1 μg/mL (100 μL/well) can bind Mouse IgE Rabbit mAb(Catalog: A23195) with a linear range of 0.06-0.45 ng/mL.
