The Recombinant Mouse IgG2B Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IgG2B biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB2064) is produced using advanced expression systems and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 27.46 kDa with an observed molecular weight of 35-45 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Mouse IgG2B Protein
SKU:
RPCB2064
Size:
20 μg , 100 μg , 500 μg
Reactivity:
Mouse
Synonyms:
Ighg2b, Igh-3, Ig gamma-2B chain C region , Immunoglobulin heavy constant gamma 2B
Tag:
N-His
Calculated MW:
27.46 kDa
Observed MW:
35-45 kDa
Protein Description:
High quality, high purity and low endotoxin recombinant Recombinant Mouse IgG2B Protein (RPT0025), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
IgG2b,Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
Recombinant Mouse IgG2B Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
