The Recombinant Mouse IL-12/IL-12A&IL-12B Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IL-12/IL-12A&IL-12B biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1046) is produced using HEK293 cells and features a C-His(IL-12a)&No tag(IL-12b) tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 22.53 kDa(IL-12A), 35.79 kDa(IL-12B) with an observed molecular weight of 28-30 kDa(IL-12A), 45 kDa(IL-12B) under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-12/IL-12A&IL-12B Protein (RPCB1046), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA.Immobilized Mouse IL-12A&IL-12B (RPCB1046) at 5 μg/mL (100 μL/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng/mL.
Measured in a cell proliferation assay using IL2-activated mouse splenocytes. The ED50 for this effect is 0.01- 0.03 ng/mL, corresponding to a specific activity of 3.3×10 7 ~ 1× 10 8 units/mg.
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED 50 for this effect is 7.3-29.2 ng/mL, corresponding to a specific activity of 3.4×10 4 ~1.4×10 5 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Recombinant Mouse IL-12/IL-12A&IL-12B Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Recombinant Mouse IL-12/IL-12A&IL-12B Protein is greater than 95% as determined by SEC-HPLC.
Recombinant Mouse IL-12A&IL-12B stimulates cell proliferation of the IL2-activated mouse splenocytes. The ED50 for this effect is 0.01- 0.03 ng/mL, corresponding to a specific activity of 3.3×107~ 1× 10 8 units/mg.
Recombinant Mouse IL-12/IL-12A&IL-12B induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 7.3-29.2 ng/mL, corresponding to a specific activity of 3.4×104~1.4×105 units/mg.
