Description
Recombinant Mouse IL-21R/CD360 Protein
The Recombinant Mouse IL-21R/CD360 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IL-21R/CD360 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1255) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 25.73 kDa with an observed molecular weight of 38-50 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse IL-21R/CD360 Protein |
| SKU: | RPCB1255 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Mouse |
| Synonyms: | CD360, NILR, IL-21 R, IL-21 receptor, IL21R, IL-21R, interleukin 21 receptor, interleukin-21 receptor, , IL21R |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 25.73 kDa |
| Observed MW: | 38-50 kDa |
| Gene ID: | 60504 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-21R/CD360 Protein (RPCB1255), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IL21R at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-21 with a linear range of 0.128-83.57 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Interleukin-21 receptor, also known as IL-21 receptor, IL-21R, Novel interleukin receptor, IL21R and NILR, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 4 subfamily. Interleukin-21 (IL-21) belongs to a family of cytokines,and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.
 | Recombinant Mouse IL-21R/CD360 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | The purity of Mouse IL-21R/Interleukin-21 Receptor Protein (RPCB1255) was greater than 90% as determined by SEC-HPLC. |
