The Recombinant Mouse IL-33 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IL-33 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0727) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.6 kDa with an observed molecular weight of 18 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-33 Protein (RPCB0727), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μM filtered solution of 20mM PB,150mM NaCl,pH7.4Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED 50 for this effect is 0.0745-0.298 ng/mL, corresponding to a specific activity of 3.36×10 6 ~1.34×10 7 units/mg.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Mouse Interleukin 33 (IL-33) is a proinflammatory cytokine which may also regulates gene transcription inproducer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines andacts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases andinduces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and alsoleads to severe pathological changes in mucosal organs.
Recombinant Mouse IL-33 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.0745-0.298 ng/mL, corresponding to a specific activity of 3.36×106~1.34×107 units/mg.
