The Recombinant Mouse IL-4 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IL-4 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0874) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 14.15 kDa with an observed molecular weight of 20 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-4 Protein (RPCB0874), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell proliferation assay using MC/9 2 mouse mast cells. The ED 50 for this effect is 2.8-11.3 pg/mL, corresponding to a specific activity of 8.85×10 7 ~3.57×10 8 units/mg.
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED 50 for this effect is 1.18-4.70 ng/mL, corresponding to a specific activity of 2.13×10 5 ~8.51×10 6 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype.
Recombinant Mouse IL-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse IL-4 Protein is greater than 95% as determined by SEC-HPLC.
Recombinant Mouse IL4 stimulates cell proliferation of HT-2 mouse T cells. The ED50 for this effect is 1.18-4.70 ng/mL, corresponding to a specific activity of 2.13×105~8.51×106 units/mg.
