Description
Recombinant Mouse Leukemia inhibitory factor/LIF Protein
The Recombinant Mouse Leukemia inhibitory factor/LIF Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Leukemia inhibitory factor/LIF biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1165) is produced using HEK293 cells and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 26.5 kDa with an observed molecular weight of 37-45 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse Leukemia inhibitory factor/LIF Protein |
| SKU: | RPCB1165 |
| Size: | 50 μg , 100 μg |
| Reactivity: | Mouse |
| Synonyms: | LIF, CDF, DIA, HILDA, MLPLI, LIF |
| Tag: | C-His&Avi |
| Expression Host: | HEK293 cells |
| Calculated MW: | 26.5 kDa |
| Observed MW: | 37-45 kDa |
| Gene ID: | 16878 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse Leukemia inhibitory factor/LIF Protein (RPCB1165), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED 50 for this effect is 35.675-142.7 ng/mL, corresponding to a specific activity of 7×10 3 ~2.8×10 4 units/mg. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.
 | Recombinant Mouse Leukemia inhibitory factor/LIF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 35.675-142.7 ng/mL, corresponding to a specific activity of 7×103~2.8×104 units/mg. |
