Myelin-Associated Glycoprotein (MAG, Siglec-4a), is a type I transmembrane glycoprotein belonging to the Siglec family. It is composed of an extracellular segment containing five Ig-like domains, a single transmembrane segment, and a cytoplasmic domain. Mouse MAG shares 95% and 99% aa sequence identity with human and rat MAG, respectively. MAG functions as an adhesion molecule during neural development. It preferentially binds to alpha -2,3-linked sialic acid terminal structures found on cell surface molecules. MAG is selectively expressed by myelinating oligodendrocytes and Schwann cells and plays an important role in axon-myelin stability. MAG is also reported to regulate the axon cytoskeleton and support the distribution of axon molecules at the nodes of Ranvier. In addition, it has been identified as a major inhibitor of neurite outgrowth.
Product Name:
Mouse MAG/Siglec-4a Recombinant Protein (RPES1084)
Product Code:
RPES1084
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Myelin-Associated Glycoprotein,MAG,Siglec-4a
Accession:
P20917
Sequence:
Gly20-Pro516
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse MAG is produced by our Mammalian expression system and the target gene encoding Gly20-Pro516 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
55.7 kDa
AP Mol Mass:
70-120 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
