OX40, also termed CD134 and TNFRSF4, is a T cell co-stimulatory molecule of the TNF receptor superfamily which plays a key role in the survival and homeostasis of effector and memory T cells. OX40 is expressed on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. The interaction between OX40 and OX40 ligand (OX40L) will occur when activated T cells bind to professional antigen-presenting cells (APCs). The T-cell functions, including cytokine production, expansion, and survival, are then enhanced by the OX40 costimulatory signals. OX40 signals are critical for controlling the function and differentiation of Foxp3+ regulatory T cells. OX40-OX40L interaction regulates T-cell tolerance, peripheral T-cell homeostasis, and T-cell-mediated inflammatory diseases.
Product Name:
Mouse OX40/TNFRSF4 Recombinant Protein (RPES0997)
Product Code:
RPES0997
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Tumor necrosis factor receptor superfamily member 4,Tnfrsf4,OX40,CD134,Txgp1
Accession:
P47741
Sequence:
Val20-Pro211
Fusion tag:
C-Fc
Activity:
Immobilized Mouse OX40L-His(Cat: PKSM041119) at 2µg/ml(100 ?l/well) can bind Mouse OX40-Fc. The ED50 of Mouse OX40-Fc is 4.204 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse OX40 is produced by our Mammalian expression system and the target gene encoding Val20-Pro211 is expressed with a Fc tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
48.1 kDa
AP Mol Mass:
65-90 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
