Description
| Product Name: | Recombinant Mouse Prostatic Acid Phosphatase Protein (Fc Tag) |
| SKU: | RPES8438 |
| Size: | 20μg |
| Tag: | C-Fc |
| Reactivity: | Mouse |
| Expression Host: | Mammalian |
| Accession: | Q8CE08 |
| Sequence: | Met1-Asn381aa |
| Calculated Molecular Weight: | 66.8 kDa |
| Observed Molecular Weight: | 80 kDa |
| Synonyms: | 5'-NT, 5'-nucleotidase, Acid phosphatase prostate, ACP 3, ACP3, acpP, Ecto-5'-nucleotidase, PAP, PPAP, Prostatic acid phosphatase, Prostatic acid phosphotase, Thiamine monophosphatase, TMPase |
| Endotoxin: | < 1.0 EU/mg of the protein as determined by the LAL method |
| Purity: | > 90% as determined by reducing SDS-PAGE. |
ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature Human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, Human ACPP shares 84% aa identity with an Mouse ACPP.
| Bio-activity: | Not validated for activity |
| Formulation: | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
