The Recombinant Mouse PVR/CD155 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate PVR/CD155 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1616) is produced using HEK293 cells and features a C-hFc&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 62.54 kDa with an observed molecular weight of 80-100 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse PVR/CD155 Protein (RPCB1616), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse CD155 at 2 μg/mL (100 μL/well) can bind Mouse TIGIT with a linear range of 0.3-4.27 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The recombinant human PVR consists of 323 amino acids with a molecular weight of 35.1 kDa.CD155 is also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5) It is a type I transmembrane single-span glycoprotein belonging to the nectins and nectin-like (Necl) subfamily. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system.Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Recombinant Mouse PVR/CD155 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Mouse CD155 at 2 μg/mL (100 μL/well) can bind Mouse TIGIT with a linear range of 0.3-4.27 ng/mL.
