Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation.
Product Name:
Mouse RANKL/TNFSF11 Recombinant Protein (RPES3368)
Product Code:
RPES3368
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Tumor necrosis factor ligand superfamily member 11,Tnfsf11,Osteoclast differentiation factor,ODF,Osteoprotegerin ligand,OPGL,Receptor activator of nuclear factor kappa-B ligand,RANKL,TNF-related activation-induced cytokine,TRANCE,CD254
Accession:
O35235
Sequence:
Ala73-Asp316
Fusion tag:
C-6His
Activity:
Immobilized Human OPG-Fc(Cat: PKSH033124) at 2µg/ml(100 ?l/well) can bind Mouse RANKL-His. The ED50 of Mouse RANK L-His is 2.44ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse TNF-related Activation-induced Cytokine is produced by our Mammalian expression system and the target gene encoding Ala73-Asp316 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
28.5 kDa
AP Mol Mass:
30-35 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
