Description
Recombinant Mouse/Rat VEGF-C Protein
The Recombinant Mouse/Rat VEGF-C Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Recombinant Mouse/Rat VEGF-C biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB2113) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 13.89 kDa with an observed molecular weight of 20-25 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse/Rat VEGF-C Protein |
| SKU: | RPCB2113 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Mouse/Rat |
| Synonyms: | VEGFC, Flt4-L, LMPH1D, VRP, VEGF-C |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 13.89 kDa |
| Observed MW: | 20-25 kDa |
| Gene ID: | 22341 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse/Rat Flt4 ligand/VEGF-C Protein (RPCB2113), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.01 EU/μg of the protein by LAL method |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Mouse/Rat VEGF-C (RPCB2113) at 2 μg/mL (100 μL/well) can bind Human VEGFR-3/FLT-4 (RPCB0299) with a linear range of 0.0085-7.49 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.
 | Recombinant Mouse/Rat VEGF-C Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Measured by its binding ability in a functional ELISA. Immobilized Mouse/Rat VEGF-C (RPCB2113) at 2 μg/mL (100 μL/well) can bind Human VEGFR-3/FLT-4 (RPCB0299) with a linear range of 0.0085-7.49 ng/mL. |
