The Recombinant Mouse TNFRSF1A/TNF-R1/CD120a Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate TNFRSF1A/TNF-R1/CD120a biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0816) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 49.98 kDa with an observed molecular weight of 60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse TNFRSF1A/TNF-R1/CD120a Protein (RPCB0816), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-alpha Protein at 0.5 μg/mL (100 μL/well) can bind Mouse TNFR1 with a linear range of 0.06-15.7 ng/mL.
Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED 50 for this effect is typically 75-300 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-α.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Mouse TNFRSF1A/TNF-R1/CD120a Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse TNFRSF1A inhibit the TNFα-mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 75-300 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-α.
