Description
Recombinant Mouse TNFRSF5/CD40 Protein
The Recombinant Mouse TNFRSF5/CD40 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate TNFRSF5/CD40 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1527) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 20.20 kDa with an observed molecular weight of 25-30 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse TNFRSF5/CD40 Protein |
| SKU: | RPCB1527 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Mouse |
| Synonyms: | CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50, CD40 |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 20.20 kDa |
| Observed MW: | 25-30 kDa |
| Gene ID: | 21939 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse TNFRSF5/CD40 Protein (RPCB1527), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins.plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines.Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3).
 | Recombinant Mouse TNFRSF5/CD40 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
